Determination of Triptonide and Triptolide in Multi-glycosides Tripterygium Wilfordii Tablets by Capillary Electrophoresis

A capillary electrophoresis method has been established for the determination of triptonide and triptolide in Multi-glycosides Tripterygium wilfordii tablets. Capillary separation was carried out by using 0.01mol L borax + 0.02 mol L boric acid + 0.02 mol L SDS (pH = 8.0) as the buffer, a fused – silica capillary tube column (60.2 cm × 75μm id. 52.6 cm of effective length) as the separation channel, 20 kV applied voltage, 301 K temperature, 5s hydrodynamic sample injection time and 214 nm UV detection wavelength. Baseline separation and good linearity were obtained under the optimum conditions. Conclusion: This method was effective and quick.